CHROMATOGRAPHIC SEPARATION OF DNP-AMINO ACIDS
- 25 February 1960
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 47 (2), 253-258
- https://doi.org/10.1093/oxfordjournals.jbchem.a127058
Abstract
The presented methods are the combination of Amberlite IRC-50 and Duolite C-25 column chromatographies by using several eluants, consisting of dilute HCl containing various amounts of acetic acid, acetone or methylethylketone. Recovery of 22 DNP-amino acids tested is 80-100%, except a low value (60-80%) for (DNP)2-ornithine. The presented methods can separate DNP-allothreonine and DNP-alloisoleucine from the respective isomers. About 0.5 mg each of DNP-amino acid can be applied per square centimeter.This publication has 7 references indexed in Scilit:
- CHROMATOGRAPHIC SEPARATION OF PHENOLIC ACIDSThe Journal of Biochemistry, 1959
- CHROMATOGRAPHIG SEPARATION OF LOWER FATTY ACIDSThe Journal of Biochemistry, 1958
- PEPTIDES OBTAINED BY TRYPTIC HYDROLYSIS OF PERFORMIC ACID-OXIDIZED RIBONUCLEASEJournal of Biological Chemistry, 1956
- Über die Receptorsubstanz für den Phagen T 5. IV. Mitteil.: Eine einfache Methode zur quantitativen Bestimmung von Aminosäuren und ihre Anwendung für Vergleiche zwischen Receptorsubstanz und mutativem AbwandlungsproduktHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1956
- Automatic fraction collectors and a conductivity recorderBiochemical Journal, 1951
- Separation of Amino-Acids as Dinitrophenyl DerivativesNature, 1951
- The fractionation of weak electrolyte mixtures by ion-exchange resinsBiochemical Journal, 1949