A SENSITIVE SIMPLE QUANTITATIVE REACTION FOR IODINATED AMINO ACIDS OF HUMAN SERUM ON PAPERCHROMATOGRAMS
- 1 January 1963
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 42 (1), 153-162
- https://doi.org/10.1530/acta.0.0420153
Abstract
A simple quantitative reaction for iodinated amino acid spots on paper- chromatograms is described. The method is a modification of the Ferric chloride, (potassium) FerricCyanide, Arseneous acid reaction (F.F.C.A.). This modified reaction has the advantages: (1) The spots obtained are well defined and well contrasted against the background. (2) Qualitative and quantitative determinations can be done simultaneously at any time. (3) The approximate sensitivity range for thyroxine is between 0.1-0.005 [mu]g. (4) More than thirty determinations can be done in 7-8 hours.Keywords
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