Enzyme immunoassay of somatostatin(SS)-like immunoreactive substance in bovine milk.
- 1 January 1990
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 38 (2), 456-459
- https://doi.org/10.1248/cpb.38.456
Abstract
A sensitive and specific enzyme immunoassay (EIA) for somatostatin (SS)-like immunoreactivity (SS-LI) was developed with the use of .beta.-D-galactosidase labeled antigen. The minimum amount of SS-like immunoreactive substance (SS-IS) detectable by this method was 1.0 fmol/well (25 pmol/l). The level of SS-IS in bovine foremilk was about 20 pmol/l, and the level was unchanged after delivery. On the other hand, the levels of gastrin releasing peptide (GRP)-IS and vasoactive intestinal polypeptide (VIP)-IS in bovine foremilk were very high, but fell during 1 week after delivery to about 10% of those in foremilk.This publication has 18 references indexed in Scilit:
- Human somatostatin I: sequence of the cDNA.Proceedings of the National Academy of Sciences, 1982
- ISOLATION AND CHARACTERIZATION OF RAT PANCREATIC SOMATOSTATINEndocrinology, 1980
- Epidermal Growth Factor Is a Major Growth-Promoting Agent in Human MilkScience, 1980
- Milk prolactin is biologically activeLife Sciences, 1980
- Isolation and Characterization of Somatostatin from Anglerfish Pancreatic Islet*Endocrinology, 1979
- Isolation and characterization of somatostatin from pigeon pancreas.Proceedings of the National Academy of Sciences, 1979
- TRANSFER OF MILK PROLACTIN TO THE PLASMA OF NEONATAL RATS BY INTESTINAL ABSORPTIONJournal of Endocrinology, 1978
- Somatostatin release from isolated perfused rat stomachBiochemical and Biophysical Research Communications, 1978
- Somatostatin in Hypothalamus, Extrahypothalamic Brain, and Peripheral Tissues of the Rat*Endocrinology, 1978
- Occurrence of somatostatin-like immunoreactivity in some peripheral sympathetic noradrenergic neuronsProceedings of the National Academy of Sciences, 1977