Segments of simian virus 40 DNA spanning most of the leader sequence of the major late viral messenger RNA are dispensable.

Abstract

A highly specific procedure for the isolation of deletion mutants is described. The size and location of the deletions can be predetermined. By this method a series of deletion mutants mapping within and near the untranslated 5'' leader sequence of the late 16S mRNA of SV 40 were isolated. The boundaries of the deletions were accurately determined by DNA sequence analysis. The deletions range from 20-223 nucleotides. All these deletion mutants are viable and grow without helper virus. The largest of these deletions removes the entire leader sequence except for 6 nucleotides at the 3'' end that are probably involved in covalent linkage with the 5'' end of the body of the mRNA located 937 nucleotides away on the genome. Three of the deletion mutants remove the 5'' end of the leader that normally bears the cap structure of the mRNA. A large segment immediately preceding the leader sequence is also removed in one of these mutants, ruling out the generation of the 5'' end of the mRNA via initiation of transcription at this point. The circularization of linear infection DNA producing the DNA of the deletion mutants proceeds mainly by way of blunt end ligation in vivo.