Membrane Enzymes: Artifacts in Arrhenius Plots Due to Temperature Dependence of Substrate-Binding Affinity
- 24 February 1978
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 199 (4331), 902-904
- https://doi.org/10.1126/science.146257
Abstract
For the membrane sodium-stimulated magnesium-adenosinetriphosphatase of Acholeplasma laidlawii B both the Vmax and Km values in the Michaelis equation very strongly with temperature. Simulations of Arrhenius plots show that an enzyme with a temperature-dependent Km can yield a variety of Arrhenius plot artifacts, most notably erroneous "breaks," if activity is assayed at a fixed substrate concentration.This publication has 11 references indexed in Scilit:
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