Abstract
A cell-free system capable of a-amylase synthesis has been obtained from the aleurone layers of germinating barley. This system requires potassium chloride, sucrose and an amino acid mixture in order to function. The crude preparation does not require calcium chloride. Chloramphenicol inhibits a-amylase synthesis as indicated both by increase in measurable enzyme activity and incorporation of L-[U-14C]glutamic acid.