The estimation of free sugars in skeletal muscle of codling (Gadus callarias) and herring (Clupea harengus)
- 1 April 1958
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 68 (4), 704-708
- https://doi.org/10.1042/bj0680704
Abstract
Ribose in extracts of herring and codling muscles reacts readily with nitrogenous constituents during application to chromatograms. Glucose is stable. Electrolytic de-salting interferes with the estimation of ribose. Sulphonated-polystyrene resins in hydrogen form hydrolyse adenosine at room temperature but the error introduced thereby in muscle analyses is insignificant. Removal of salt and amino nitrogen by suitable mixed ion-exchange resins followed by lyophilizing and quantitative chromatog-raphy gave an estimate of glucose and ribose in codling and herring accurate to [plus or minus]6%.Keywords
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