Activity Curves of Crude and Purified Inhibitors and Accelerators of Blood Coagulation.

Abstract

Crude lipid extractions of human brain when tested on stable citrated plasma in collodion tubes display curves of activity of a biphasic nature, when a wide range of concn. of the extracts is used. This seems to reflect the coexistence in the extracts of both inhibitors and accelerators of clotting. Purified fractions of the extracts yield on testing monophasic curves of activity, demonstrating perhaps that the extract has been freed of antagonists by the purification. Normal blood and plasma behave on dilution like a mixture of coagulants and anticoagulants. Hemophilic blood and plasma behave on dilution like a predominantly anticoagulant mixture, while post-hemorrhagic hypercoagulable blood and plasma display curves of activity resembling a predominantly coagulant mixture. Excepting Fraction V, all Cohn''s plasma fractions have coagulant action; only Fraction IV-1 and IV-4 display a biphasic curve of activity, an indication that they are mixtures of coagulants and anticoagulants.