SITE-SPECIFIC OSO4 MODIFICATION OF THE B-Z JUNCTIONS FORMED AT THE (DA-DC)32 REGION IN SUPERCOILED DNA
- 25 May 1986
- journal article
- research article
- Vol. 261 (15), 7093-7098
Abstract
OsO4 in the presence of pyridine specifically modifies the structural distortions of the primary helix of supercoiled pRW777 near the (dA-dC)32 sequence. Modification occurs at the same negative superhelix density value as required for formation of the Z-helix within the polymer block. Fine mapping of the distorted regions, which are probably the B-Z junctions, is presented. OsO4 reactions provide a powerful and sensitive chemical approach to study DNA polymorphism in solution.This publication has 28 references indexed in Scilit:
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