Combined Platelet‐Leukapheresis: A Technique for Preparing Separate Platelet and Granulocyte‐Platelet Units from Single Donors

Abstract
One donor can provide separate platelet and granulocyte-platelet units that have the potential to fulfill the needs of 2 recipients. Combined platelet and leukapheresis were performed in the presence of hydroxyethyl starch and citrate employing the Hemonetics Model 30 Blood Separator. Platelets and granulocytes were separated subsequently by centrifugation and 2 individual units were prepared for transfusion. The platelet units contained a mean of 4.4 .times. 1011 platelets/unit and were nearly devoid of leukocytes. The combined granulocyte-platelet units contained a mean of 7.38 .times. 109 neutrophils/unit and 3.06 .times. 1011 platelets/unit. Thus, 2 separate units containing sufficient numbers of cells for transfusion can be obtained from pheresis of 1 donor.