Characteristics of the T‐dependent α(1 → 6) glucosyl (dextran) antibody response induced in mice with isomaltohexaose coupled to chicken γ‐globulin

Abstract

Isomaltohexaose flavazole coupled to chicken γ‐globulin (IM6‐CGG) induced T celldependent anti‐α( 1 → 6) dextran‐specific IgM and IgG responses in CBA, BALB/c and A strain mice. The IgG responses were of restricted heterogeneity as judged by isoelectric focusing, and belonged mostly to the IgG₁ subclass with a minor IgG₃ component in the case of BALB/c and CBA mice. All four subclasses of IgG were produced in A strain mice. In contrast, native dextran B 512 induces exclusively T cell‐independent IgM responses of the same specificity for all 3 strains. All BALB/c mice immunized with different doses of IM6‐CGG either in Freund's adjuvant or with A1(OH)₃ plus Bordetella pertussis showed the same spectrotypes by isoelectric focusing. Sera obtained at different times after immunization of BALB/c mice failed to show spectrotype variations. Late, but not early, bleedings from CBA mice showed a tendency towards more uniform isoelectric focusing patterns.