COLLECTION AND PROCESSING OF BLOOD-SAMPLES DRIED ON PAPER FOR MICRO-ASSAY OF NEWCASTLE-DISEASE VIRUS AND AVIAN INFLUENZA-VIRUS ANTIBODIES
- 1 January 1980
- journal article
- research article
- Vol. 41 (9), 1495-1499
Abstract
A practical method for collection and processing of dried whole [chicken] blood samples on filter paper was developed to facilitate large-scale testing programs for Newcastle disease virus and avian influenza virus antibodies. A modified paper punch was used to cut and place dried blood samples simultaneously in a standard 96-well microplate for elution of antibody. Twelve eluted samples were simultaneously transferred to another microplate for the hemagglutination-inhibition (HI) microtest. Similar HI titers were obtained with simultaneously collected serum and dried blood samples. Minor differences were not considered of practical importance in diagnostic serologic studies. Dried blood titers were not markedly affected by method of drying (37.degree. C for 2 h or 26.degree. C for 4 h), by storage for 24 h before drying or by storage of dried samples at 4.degree. C for 28 days or 30.degree. C for 14 days. Blood dried on paper was a satisfactory sample for assay on HI antibodies to Newcastle disease virus and avian influenza virus.This publication has 6 references indexed in Scilit:
- IMMUNIZATION OF CHICKENS AND TURKEYS AGAINST AVIAN INFLUENZA WITH MONO-VALENT AND POLYVALENT OIL EMULSION VACCINES1979
- A Simple Method for Recording and Analyzing Serological DataAvian Diseases, 1978
- The Influence of Test Conditions on Newcastle Disease Hemagglutination-Inhibition TitersAvian Diseases, 1978
- Use of the Nobuto Blood-Sampling Paper Strip for Newcastle Disease SerologyAvian Diseases, 1977
- Use of Dried Whole Blood Collected on Filter Paper Disks in Adenovirus Complement Fixation and Measles Hemagglutination Inhibition TestsThe Journal of Immunology, 1964
- A PROCEDURE FOR ADSORBING VIRUS NEUTRALIZING ANTIBODIES ON PAPER DISKSJournal of Bacteriology, 1956