COLLECTION AND PROCESSING OF BLOOD-SAMPLES DRIED ON PAPER FOR MICRO-ASSAY OF NEWCASTLE-DISEASE VIRUS AND AVIAN INFLUENZA-VIRUS ANTIBODIES

Abstract

A practical method for collection and processing of dried whole [chicken] blood samples on filter paper was developed to facilitate large-scale testing programs for Newcastle disease virus and avian influenza virus antibodies. A modified paper punch was used to cut and place dried blood samples simultaneously in a standard 96-well microplate for elution of antibody. Twelve eluted samples were simultaneously transferred to another microplate for the hemagglutination-inhibition (HI) microtest. Similar HI titers were obtained with simultaneously collected serum and dried blood samples. Minor differences were not considered of practical importance in diagnostic serologic studies. Dried blood titers were not markedly affected by method of drying (37.degree. C for 2 h or 26.degree. C for 4 h), by storage for 24 h before drying or by storage of dried samples at 4.degree. C for 28 days or 30.degree. C for 14 days. Blood dried on paper was a satisfactory sample for assay on HI antibodies to Newcastle disease virus and avian influenza virus.