Semisynthesis and biological properties of the [B24-leucine]-, [B25-leucine]- and [B24-leucine, B25-leucine]-analogues of human insulin
- 1 August 1981
- journal article
- research article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 37 (8), 811-813
- https://doi.org/10.1007/bf01985653
Abstract
Trypsin-catalyzed coupling of porcine desoctapeptide-insulin with synthetic octapeptides produced the [LeuB24]-(I), [LeuB25]- (II) and [LeuB24, LeuB25]- (III)analogues of human insulin. I, II and III displayed respectively 20–30%, 1–2% and 0.5% of the receptor binding activity of the normal hormone. Biological activities of these analogues seemed to be proportional to their binding potencies when assayed in vitro, while in an in vivo assay analogue I was fully active and II exhibited 10–20% of normal activity. III was less active than II in all assays tested.This publication has 16 references indexed in Scilit:
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