Trace Analysis of Estradiol in Animal Chow by Electron-Capture Gas Chromatography
- 1 May 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 15 (5), 160-183
- https://doi.org/10.1093/chromsci/15.5.160
Abstract
A gas chromatographic method is described for trace analysis of the natural steroidal hormone estradiol in animal chow at levels as low as 3 ppb. Salient elements of the method include extraction of the estradiol with methanol, an initial clean-up on a column of Sephadex LH-20, liquid-liquid partitioning at pH 14 and 10.2, additional cleanup on a silica gel column, conversion of the estradiol to the pentafluoropropionyl (PFP) derivative and analysis by electron-capture gas chromatography on a column of OV-25. Samples containing less than 100 ppb of estradiol are subjected to further cleanup on silica gel after derivatization and prior to analysis.This publication has 5 references indexed in Scilit:
- Chromatographic analysis of hormone residues in foodJournal of Chromatography A, 1976
- GAS CHROMATOGRAPHIC AND MASS SPECTROMETRIC STUDIES IN INFLUENCE OF METABOLITES OF BIS(P-ACETOXYPHENYL)CYCLOHEXLIDENEMETHANE ON ESTIMATION OF ESTROGENS IN URINE1967
- A chemical method for the determination of oestriol, oestrone and oestradiol in human urineBiochemical Journal, 1955
- Separation of oestrogens in urinary extracts by partition chromatographyBiochemical Journal, 1955
- THE MEASUREMENT OF ESTROGEN MIXTURES BY DIFFERENTIAL FLUOROMETRY1,2Endocrinology, 1953