Single-Feature Inking and Stamping: A Versatile Approach to Molecular Patterning

Abstract
A technique for micrometer-scale patterning of multiple functional biological molecules on surfaces is demonstrated. The technique is referred to as single-feature inking and stamping (SFINKS). It combines elements of dip-pen nanolithography and microcontact printing. “Inked” atomic force microscopy probes are used to ink individual features of an elastomer stamp. From a single stamp, we printed three different probe ssDNA with 100 μm and composed of 2 μm lines. We discuss why patterns such as these are impractical with available techniques. Furthermore, we comment on the prospects for multiple stamping after a single inking.