Extraction and Concentration of Thromboplastic Material from Human Urine

Abstract

Thromboplastic material together with fibrinolysokinase are contained in normal human urine and are adsorbed on BaSO4 at pH 4.5. The thromboplastic material, but not the fibrinolysokinase, is eluted with H2O at pH 5.2. After dialysis the thromboplastic material is precipitated at pH 3.5, redissolved in 1/100 [image] glycine buffer pH 9, centrifuged 30 minutes at 2000 g, dialyzed and lyophilized. A few ug/cc of the material normalizes the clotting and prothrombin serum time of hemo-philic plasma. The material can replace the platelets in the thromboplastin generation test. It is rather heat resistant and becomes fully activated by incubation with serum and BaSO4-plasma. It is soluble in 1/100 [image] glycine buffer at pH 9 and is precipitated by prolonged highspeed centrifugation.