An in vivo comparison of CPD and CPDA‐2 preserved platelet concentrates after an 8‐hour preprocess hold of whole blood

Abstract

To see if citrate-phosphate-dextrose-adenine-2 (CPDA-2) anticoagulant-preservative had an effect on the viability of platelets, autologous in vivo recovery and survival in humans for platelet concentrates prepared from 6 U of blood drawn into CPDA-2 were studied and compared to those of 6 U drawn into citrate-phosphate-dextrose (CPD). These units were prepared from whole blood held at room temperature for 8 h after collection and then were stored for 3 days at 22.degree. .+-. 22.degree. C. The recovery for platelets preserved in CPD was 39.0 .+-. 4.8%, and for platelets preserved in CPDA-232.5 .+-. 4.4%. The difference was not significant (P > 0.10). To estimate population differences, in vitro effects on in vivo viability were also evaluated. Six in vitro variables were studied but only pH at 72 h (r = 0.77), platelet count (r = 0.64) and morphology score (r = 0.66) correlated to recovery. Only pH at 72 h significantly influenced recovery (P = 0.007). By adjusting for individual pH differences, mean recovery for platelets stored in CPD was 37.5%, and for platelets stored in CPDA-234.0%. The mean lifespan was 6.7 .+-. 0.7 days for platelets preserved in CPD and 6.1 .+-. 1.0 days for those preserved in CPDA-2. Although hemostatic function was not studied, these data support in vitro observations that platelets preserved with CPDA-2 are not different from platelets preserved with CPD, even after 8-h of storage of whole blood at room temperature prior to platelet concentrate preparation.