Analysis of NZB hyperdiploid spleen cells.
- 1 January 1981
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 126 (1), 154-160
- https://doi.org/10.4049/jimmunol.126.1.154
Abstract
In order to study the cell types involved in the hyperdiploidy characteristic of NZB mice, flow cytometric techniques, which measure te DNA content of individual cells, have been used together with standard cytogenetic analysis. The aneuploid cells present in NZB mice were found to be clonally derived. These cells were of large size relative to nonaneuploid cells. They could not be removed by lysis with anti-Thy 1 plus complement nor were they present in the nylon-wool column nonadherent fraction, both of which are characteristic of T cells. By further cytotoxic analysis, the aneuploid cells were found not to express Ly-5 nor NK surface antigens, found on natural killer cells. The aneuploid cells had increased quantities of cell surface H-2 antigen. This marked susceptibility to lysis with anti-H-2 serum was associated with one or more extra copies of chromosome 17, which carries the H-2 complex. A large proportion of these cells also expressed surface Ia and Ig. The aneuploid cells found in the spleens of older NZB mice derive from bone marrow stem cells. Neonatally thymectomized and lethally irradiated NZB X DBA/2 recipients of anti-Thy-1 treated young NZB bone marrow cells ultimately developed aneuploid spleen cells. Such cells are not found in intact or thymectomized NZB Z DBA/2 mice nor in recipients of DBA/2 marrow. Finally, congenic NZB mice carrying the CBZ/N xid failed to develop aneuploidy. Taken together, these studies suggest that the aneuploid cell is a bone marrow-derived stem cell destined to differentiate into the B cell subset lacking in CBA/N mice.This publication has 14 references indexed in Scilit:
- Regulation of Cytotoxic Lymphocyte Responses in New Zealand Mice by Alloantigen-Activated Spleen CellsThe Journal of Immunology, 1979
- Distribution of lymphocytes identified by surface markers in murine strains with systemic lupus erythematosus-like syndromes.The Journal of Experimental Medicine, 1979
- Neoplastic behavior of chromosomally abnormal clones in New Zealand black miceInternational Journal of Cancer, 1978
- Longitudinal studies of chromosomal abnomalities and reticulum cell proliferation in new zealand black miceInternational Journal of Cancer, 1978
- Specificity of acquired clonal chromosome abnormalities in new zealand black miceInternational Journal of Cancer, 1978
- Flow Microfluorometric Analysis of P3SS Murine Leukemia After Administration of Vincristine and Maytansine In VivoJNCI Journal of the National Cancer Institute, 1978
- TRANSPLANTATION OF AUTO-IMMUNE POTENTIAL .3. IMMUNOLOGICAL HYPER-RESPONSIVENESS AND ELEVATED ENDOGENOUS SPLEEN COLONY FORMATION IN LETHALLY IRRADIATED RECIPIENTS OF NZB BONE-MARROW CELLS1978
- Demonstration of Activation of B Lymphocytes in New Zealand Black Mice at Birth by an Immunoradiometric Assay for Murine IgMThe Journal of Immunology, 1977
- B-lymphocyte heterogeneity: development and characterization of an alloantiserum which distinguishes B-lymphocyte differentiation alloantigens.The Journal of Experimental Medicine, 1977
- Suppression of autoimmunity in nzb mice with steroid‐sensitive x‐radiation‐sensitive syngeneic young thymocytesArthritis & Rheumatism, 1976