Design of Hammerhead Ribozymes to Distinguish Single Base Changes in Substrate RNA
- 1 January 1996
- journal article
- research article
- Published by Mary Ann Liebert Inc in Antisense and Nucleic Acid Drug Development
- Vol. 6 (3), 165-168
- https://doi.org/10.1089/oli.1.1996.6.165
Abstract
Hammerhead ribozymes are attractive tools in antisense gene inactivation because of their catalytic cleavage of target molecules. High sequence discrimination should be possible, since the cleavage efficiencies were already significantly reduced, if single base changes in substrate RNA introduce mismatches next to the cleavage site. This was observed at the first innermost base pair in helix I and the two innermost base pairs in helix III. In addition to its position, the nature of the mismatch pair was important.Keywords
This publication has 21 references indexed in Scilit:
- Similar cleavage efficiencies of an oligoribonucleotide substrate and an mdr1 mRNA segment by a hammerhead ribozymeGene, 1995
- Towards a new concept of gene inactivation: specific RNA cleavage by endogenous ribonuclease PBiotechnology Annual Review, 1995
- Suppression of H-ras-mediated transformation in NIH3T3 cells by a ras ribozymeBiochemical Pharmacology, 1994
- Efficient cleavage of pre-tRNAs byE.coliRNAse P RNA requires the 2′-hydroxyl of the ribose at the cleavage siteNucleic Acids Research, 1993
- Implications of ribozyme kinetics for targeting the cleavage of specific RNA molecules in vivo: more isn't always better.Proceedings of the National Academy of Sciences, 1991
- Design of RNA enzymes distinguishing a single base mutation in RNANucleic Acids Research, 1989
- Cleavage of specific sites of RNA by designed ribozymesFEBS Letters, 1988
- Simple RNA enzymes with new and highly specific endoribonuclease activitiesNature, 1988
- Non-enzymatic cleavage and ligation of RNAs complementary to a plant virus satellite RNANature, 1986
- Self-cleavage of plus and minus RNA transcripts of avocado sunblotch viroidNucleic Acids Research, 1986