SOME PROPERTIES OF AN ALKALINE PHOSPHATASE FROM RAT ADIPOSE TISSUE
- 1 October 1964
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 42 (10), 1445-1457
- https://doi.org/10.1139/o64-156
Abstract
An alkaline phosphatase was partially purified from aqueous extracts of rat adipose tissue, and some characteristics of the enzyme delineated. Among salient features, the enzyme is strongly inhibited by cysteine and ethylene diaminetetraacetic acid (EDTA) and types of inhibition were determined. Cysteine induced "complete exclusive" inhibition, while EDTA caused "partial" inhibition. Kinetic data on the inhibition by EDTA are consistent with a mechanism which does not involve chelation of a metallic cation. Various other properties of the enzyme are discussed.Keywords
This publication has 9 references indexed in Scilit:
- REPRESSION OF ALKALINE PHOSPHATASE IN HUMAN CELL CULTURES BY CYSTINE AND CYSTEINEProceedings of the National Academy of Sciences, 1963
- Alkaline phosphatase activity of human cell cultures: Kinetic and physical-chemical propertiesArchives of Biochemistry and Biophysics, 1962
- α-Glycerophosphate Synthesis and Breakdown in Homogenates of Adipose TissuePublished by Elsevier ,1962
- ALPHA-GLYCEROPHOSPHATE SYNTHESIS AND BREAKDOWN IN HOMOGENATES OF ADIPOSE TISSUE1962
- SYNTHESIS OF PHOSPHATIDES IN ISOLATED MITOCHONDRIAPublished by Elsevier ,1954
- SYNTHESIS OF PHOSPHATIDES IN ISOLATED MITOCHONDRIA .3. THE ENZYMATIC PHOSPHORYLATION OF GLYCEROL1954
- ROLE OF METAL IONS IN ENZYME SYSTEMSPhysiological Reviews, 1950
- Beziehungen der Glycerophosphatase zur Kohlehydrat-Fettsynthese im FettgewebeNaunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie, 1939
- The Determination of Enzyme Dissociation ConstantsJournal of the American Chemical Society, 1934