Evidence for a calcium activated protease specific for lens intermediate filaments
- 1 January 1984
- journal article
- research article
- Published by Taylor & Francis in Current Eye Research
- Vol. 3 (3), 423-429
- https://doi.org/10.3109/02713688408997229
Abstract
The calcium mediated loss of intermediate filament protein from lens cytoskeletal preparations was examined by soft laser scanning densitometry of polyacrylamide gels. The time course of proteolysis by the lens Ca++ activated proteinase and inhibition by EGTA or PMSF and leupeptin were also determined. Proteolytic breakdown products were identified on eleotroblots with specific antiserum to vimentin.This publication has 27 references indexed in Scilit:
- Neurofilament Accumulation Induced in Synapses by LeupeptinScience, 1983
- The turnover of vimentin in Ehrlich ascites tumour cellsFEBS Letters, 1983
- In vivo metabolism of mammalian neurofilament polypeptides in developing and adult rat brainFEBS Letters, 1982
- Characterization of a brain calcium-activated protease that degrades neurofilament proteinsBiochemistry, 1982
- Intermediate Filaments: A Chemically Heterogeneous, Developmentally Regulated Class of ProteinsAnnual Review of Biochemistry, 1982
- Synthesis of cytoskeletal and membrane polypeptides by the organ cultured chicken lensCurrent Eye Research, 1982
- The cytoskeleton of chick lens cellsExperimental Eye Research, 1979
- Localization of creatine kinase isoenzymes in myofibrils. II. Chicken heart muscle.The Journal of cell biology, 1977
- Regional Differences in the Polypeptide Composition of Chick Lens Intracellular MatrixOphthalmic Research, 1977
- A calcium(2+) ion-activated protease possibly involved in myofibrillar protein turnover. Partial characterization of the purified enzymeBiochemistry, 1976