The codon CUG is read as serine in an asporogenic yeast Candida cylindracea

Abstract

DEVIATIONS from the universal genetic code have been reported for several microorganisms. Termination codons are used for cod-ing some amino acids in Paramecium^1,2, Mycoplasma³ or Tetrahymena^4,5, and in Escherichia coli, the UGA termination codon is used to code for selenocysteine⁶. In mitochondria, the changes of sense codons to termination codons or to codons encoding other amino acids have also been reported^7,8. Here we report another example of divergence from the universal code, this time in a non-spore-forming yeast Candida cylindracea, in which the uni-versal codon for leucine, CUG, is used to code for serine. This conclusion is based on the observations that: (1) the amino-acid composition and the partial amino-acid sequences of an extracel-lular lipase from this yeast agreed with those deduced from the complementary DNA if CUG was assumed to specify serine; and (2) serine, but not leucine, was incorporated into a polypeptide in a cell-free translation system from this yeast in the presence of a synthetic CUG oligomer.