THE DETERMINATION OF 17-KETOGENIC STEROIDS

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Abstract
A study of the kinetics of the oxidation of 17-hydroxycorticosteroids to 17-ketosteroids (17-KS) by sodium bismuthate shows that the oxidation of the corticoid side chain is more rapid in aqueous ethanolic solution containing chlorosubstituted acetic acids than in aqueous acetic acid solution. Conditions for oxidation of 17-hydroxycorticosteroids in trichloroacetic acid (0.25 [image]) were established to give quantitative yields of 17-KS from 17, 20, 21-triols, 17, 21-diol-20-ketones and 17, 20-diols. Based on these results a method was developed for the determination of 17-ketogenic steroid (17-KGS) glucuronides in urine. Theoretical recovery of added 17-KGS and androsterone glucuronide indicates that little, if any, 17-KGS is conjugated as sulfate during the course of the procedure.