Organic-Phase Enzyme Electrode for the Determination of Phenols in Olive Oils
- 1 August 1992
- journal article
- biosensors
- Published by Taylor & Francis in Analytical Letters
- Vol. 25 (8), 1399-1409
- https://doi.org/10.1080/00032719208017124
Abstract
A rapid procedure for determining phenols in olive oils, based on an organic-phase enzyme electrode, is described. Direct assays are performed in chloroform solutions which support the tyrosinase activity. This class (phenol)-specific enzyme strongly adheres to the surface of the graphite transducer. The resulting wall-jet detector offers effective flow injection operation, with a detection limit of 4×10−7 M (0.8 ng) phenol and sample frequency of 60 h−1. Applicability to olive-oil samples of different origins is illustrated.Keywords
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