COVALENT STRUCTURE OF CALF SKIN TYPE-III COLLAGEN .2. AMINO-ACID SEQUENCE OF THE CYANOGEN-BROMIDE PEPTIDE ALPHA-1(III)CB1,8,10,2(POSITIONS 223-402)

Abstract

The CNBr peptide .alpha.1-(III)CB1,8,10,2 is 180 amino acid residues in length and occupies position 223-402 along the .alpha.1(III) chain. To elucidate its amino acid sequence, .alpha.1(III)CB1,8,10,2 was fragmented with hydroxylamine, protease from Staphylococcus aureus V8 and trypsin [EC 3.4.21.4]. Peptides necessary for sequence analysis with the automated Edman degradation were separated using molecular and ion exchange chromatography. Edman degradation of the hydroxylamine-derived fragments resulted in the elucidation of 80% of the entire sequence. The rest was completely established by sequence analysis of some protease V8 and trypsin-derived peptides.