Solid phase synthesis of oligoribonucleotides using o-nitrobenzyl protection of 2′-hydroxyl via a phosphite triester approach
Open Access
- 1 January 1986
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 14 (15), 6265-6279
- https://doi.org/10.1093/nar/14.15.6265
Abstract
The hepta and undecaribonucleotide were synthesized on a controlled pore glass beads using o-nitrobenzyl protection of 2′-hydroxyls via a phosphite approach. By using 5-p-nitrophenyltetrazole for the activation of nucleoside-phosphoramidite, the condensation reaction was carried out very rapidly (2.5min). The time required for one cycle was only 16min. The hepta-(UACUAAC) and undecaribonucleotides(GUAUGUUAAUA) were obtained in yields of 28 and 17% respectively from the original resin.Keywords
This publication has 14 references indexed in Scilit:
- The "Spliceosome": Yeast Pre-Messenger RNA Associates with a 40 S Complex in a Splicing-Dependent ReactionScience, 1985
- The RNA moiety of ribonuclease P is the catalytic subunit of the enzymeCell, 1983
- Evidence for an intron-contained sequence required for the splicing of yeast RNA polymerase II transcriptsCell, 1983
- Improvements in the phosphoramidite procedure for the synthesis of oligodeoxyribonucleotidesNucleic Acids Research, 1983
- Syringe method for stepwise chemical synthesis of oligonucleotidesNucleic Acids Research, 1982
- A catalogue of splice junction sequencesNucleic Acids Research, 1982
- Studies on transfer ribonucleic acids and related compounds. XXXIX. Chemical synthesis of heptadeca- and hexadecaribonucleotides corresponding to the 3'-terminus of the tRNAfMet of E. coli.CHEMICAL & PHARMACEUTICAL BULLETIN, 1981
- Studies on the Sequence of the 3′‐Terminal Region of Turnip‐Yellow‐Mosaic‐Virus RNAEuropean Journal of Biochemistry, 1977
- Synthesis of thymidine oligonucleotides by phosphite triester intermediatesJournal of the American Chemical Society, 1976
- DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mappingNucleic Acids Research, 1974