QUATERNARY AMMONIUM COMPOUNDS IN CONNECTIVE TISSUE HISTOCHEMISTRY: I. SELECTIVE UNBLOCKING

Abstract
Certain features of an accepted biochemical system for fractionating biological polyanions were adapted for more precise identification of acid mucopolysaccharides in histological preparations. Tissue studies were based on two major steps: (1) complete blocking of all polyanions by a quaternary ammonium detergent and (2) selective unblocking by increasing concentrations of an electrolyte. Critical stages were visualized by suitable stains for acid mucopolysaccharides.