Chemosensitivity of human bladder cancer cells in long-term culture and clinical responses to the selected anticancer drug

Abstract

In vitro sensitivity of an established cell line [T24] from human urinary bladder cancer to various chemotherapeutic agents was determined by 14C-leucine incorporation into the target cells. Carboquone, neocarzinostatin, actinomycin D, adriamycin, Mitomycin C and chromonomycin A3 produced intensive cytotoxic effects, while thio-tepa, bleomycin, 5-fluorouracil and vincristine were less cytotoxic. Intravesical instillation of carboquone, one of the most toxic agents in vitro, resulted in complete or partial tumor remission in 6 of 9 patients with bladder cancer. Prophylactic effects of periodic intravesical Carboquone were also indicated in 7 of 8 patients who had experienced recurring superficial bladder tumors.