Establishment of Cell Lines in vitro from a Mammary Ascites Tumor of Mouse and Biological Properties of the Established Lines in a Serum Containing Medium
- 1 January 1966
- journal article
- research article
- Published by Tohoku University Medical Press in The Tohoku Journal of Experimental Medicine
- Vol. 88 (1), 69-84
- https://doi.org/10.1620/tjem.88.69
Abstract
Two cell lines were established in vitro from a mouse ascites mammary carcinoma FM3A and they were similar to each other both in their in vitro growth rate and their morphological identity with in vivo tumor cells. Both A and B lines has 3 characteristic chromosomes and high transplantability, and the transplanted subcutaneous tumors showed the morphology of undif-ferentiated adenocarcinoma. The cloning of the present cell lines were made with the use of agar. The study of the nutritional requirement of the present lines showed that Bactopepton, pyruvate and serine were growth enhancing respectively and the combination of these additives were more effective than single addition. The glucose utilization of the lines in the course of the growth was enhanced at the growth stage of the lag phase and early logarithmic phase but decreased after the stage of late logarithmic phase. The capacity to grow in vitro without lag time could be maintained for a long time after transplantion to the host animal, but it fell gradually.This publication has 10 references indexed in Scilit:
- LOSS OF NEOPLASTIC PROPERTIES IN VITRO .I. OBSERVATIONS WITH S-180 CELL LINES1964
- Long-term Cultivation of Pseudo-diploid Cells of Human Tumor OriginThe Tohoku Journal of Experimental Medicine, 1964
- QUANTITATIVE STUDIES OF THE GROWTH OF MOUSE EMBRYO CELLS IN CULTURE AND THEIR DEVELOPMENT INTO ESTABLISHED LINESThe Journal of cell biology, 1963
- IN VITRO CULTIVATION OF MN-LYMPHOSARCOMA CELLS1963
- Establishment of Long-Term Cultures of Ehrlich Ascites Tumor Cells.Experimental Biology and Medicine, 1962
- Long-Term Cultivation in Tissue Culture of Leukemic Cells From Mouse Leukemia Induced by Moloney Virus or by X Rays2JNCI Journal of the National Cancer Institute, 1961
- Sarcomatous Change and Maintenance of Differentiation in Long-Term Cultures of Mouse Mammary CarcinomaJNCI Journal of the National Cancer Institute, 1961
- Chromosome preparations of leukocytes cultured from human peripheral bloodExperimental Cell Research, 1960
- In vitro Culture of Ehrlich Ascites Tumor CellsScience, 1960
- Massive Fluid-Suspension Cultures of Certain Mammalian Tissue Cells. II. Glucose Utilization and Cell Proliferation23JNCI Journal of the National Cancer Institute, 1958