The further construction of the two‐dimensional database of common human proteins
- 1 January 1995
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 16 (1), 831-840
- https://doi.org/10.1002/elps.11501601138
Abstract
The master two-dimensional gel database (D. Burggraf et al., Electrophoresis 1992, 13, 729–732) [1] of common human proteins has been expanded to include detailed protein characteristics. Human cellular proteins from 5 cell lines and different cell organelles representing various tissues (muscle, nervous, connective, epithelial blood) and germ layers (ectoderm, endoderm and mesoderm), were separated by two-dimensional gel electrophoresis (2-DE). According to a recently developed algorithm, master gels of these different cells were established by computer-aided image processing. An expanded map with protein-chemical information of the polypeptides common to all human cells is shown. The synthetic, common human protein-map represents 856 spots resolved with an accuracy of 4 cm/pI unit. The protein spots were characterized either by their isoelectric point, molecular mass, integrated intensity, background-corrected optical density, spot area, or cellular distribution. About 80 proteins were further characterized and identified by protein name, amino acid composition analysis, N-terminal sequencing, enzymatic digest and subsequent peptide sequencing. Additionally the proteins of the common human protein map were identified by Western blotting. Specific information regarding glycosylation and quantitation of expression levels after chemical, biological and mechanical stimulation is included in the database.Keywords
This publication has 25 references indexed in Scilit:
- The human keratinocyte two‐dimensional protein database (update 1994): Towards an integrated approach to the study of cell proliferation, differentiation and skin diseasesElectrophoresis, 1994
- Expression of tumor‐associated protein variants in chemically induced rat hepatomas and transformed rat liver cell lines determined by two‐dimensional electrophoresisElectrophoresis, 1994
- Gel electrophoretic analysis of nuclear matrix fractions isolated from different human cell linesElectrophoresis, 1994
- Detection of the hypusine‐containing protein (HP = eIF‐5A) in crude yeast extracts by two‐dimensional Western blotsElectrophoresis, 1992
- Towards a two‐dimensional database of common human proteinsElectrophoresis, 1992
- The rat liver epithelial (RLE) cell protein databaseElectrophoresis, 1991
- A strategy for founding a global lymphocyte proteinpaedia and gene catalogueImmunology Today, 1990
- Combination of two‐dimensional gel electrophoresis with microsequencing and amino acid composition analysis: Improvement of speed and sensitivity in protein characterizationElectrophoresis, 1990
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970