The liberation of glucuronic acid from conjugated glucuronides by β-glucuronidase

Abstract

The reducing material was formed when menthylglucuronide was incubated with purified beef spleen. [beta]-Glucuronidase was identified as glucuronic acid and hence the action of the enzyme was limited to hydrolysis of the glucosidic linkage. This was in contrast to the results of Lipschitz and Bueding (see B. A. 13(7): entry 12782) which suggested that synthesis of glucuronides in tissue culture was not from glucuronic acid.