URINARY ADRENOCORTICAL STEROIDS: II. FORMALDEHYDOGENIC AND ACETALDEHYDOGENIC SUBSTANCES IN EXTRACTS OF HOT ACID-HYDROLYSED URINE

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Abstract
Formaldehydogenic (FSS) and acetaldehydogenic (ASS) substances occur in alkali-washed CHCl3 urinary extracts after boiling with 20% vol./vol. concn. HC1. This treatment results in the formation of FSS artifacts from 17-hydroxylated corticosteroids (cortisone). The admn. of cortisone increases urinary acid-hydrolyzed FSS. Probably the FSS of hot-acid-hydrolyzed urine are largely artifacts from 21-C 17-hydroxy-steroids, and not a reflection of 17-desoxy "corticosterone-like" steroids. The great lability of known urinary steroids to ASS formation in hot acid makes it doubtful that ASS-activity is derived from the adrenal cortical steroids, although the ASS content is increased after cortisone or ACTH.