Kinked DNA in crystalline complex with EcoRI endonuclease
- 24 May 1984
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 309 (5966), 327-331
- https://doi.org/10.1038/309327a0
Abstract
The 3 Å electron density map of a co-crystalline recognition complex between EcoRI endonuclease and the oligonucleotide TCGCGAATTCGCG reveals that a tight, complementary interface between the enzyme and the major groove of the DNA is the major determinant of sequence specificity. The DNA contains a torsional kink and other departures from the B conformation which unwind the DNA and thereby widen the major groove in the recognition site.Keywords
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