Tc-99m labeled leukocytes: preparation and use in identification of abscess and tissue rejection.
- 1 September 1983
- journal article
- research article
- Published by Radiological Society of North America (RSNA) in Radiology
- Vol. 148 (3), 827-831
- https://doi.org/10.1148/radiology.148.3.6308712
Abstract
A simple and reproducible method for the preparation and labeling of leukocytes with 99mTc was developed. Leukocytes were separated from blood, incubated with stannous pyrophosphate, and then with 20-30 mCi (740-1110 MBq) of 99mTcO4-. In leukocytes separated from human blood, the labeling efficiency was 81% .+-. 6% (n = 4). Experiments on dogs with abscesses showed accumulation of the Tc-99m-labeled leukocytes in the infected sites, indicating the viability of the labeled leukocytes. Additional studies showed that rat lymphocytes that were labeled with 99mTc, using the same technique, localized in heart transplant tissue that was being rejected.This publication has 9 references indexed in Scilit:
- Detection of cardiac transplant rejection with radiolabeled lymphocytes.Circulation, 1982
- MONITORING OF CARDIAC ANTI-REJECTION THERAPY WITH LYMPHOCYTES-IN-1111982
- Factors influencing the rate of 111indium-labelled lymphocytes after transfer to syngeneic ratsJournal of Immunological Methods, 1981
- Technetium-99m-labeled mouse-spleen leukocytes: Radiolabeling parametersThe International Journal of Applied Radiation and Isotopes, 1979
- CELL-DAMAGE RESULTING FROM THE LABELING OF RAT LYMPHOCYTES AND HELA S3 CELLS WITH IN-111 OXINE1979
- The Radiolabeling of Lymphocytes and Tumor Cells with 111IndiumExperimental Biology and Medicine, 1978
- IN-111-LABELED LEUKOCYTES FOR LOCALIZATION OF ABSCESSES - PREPARATION, ANALYSIS, TISSUE DISTRIBUTION, AND COMPARISON WITH GA-67 CITRATE IN DOGS1977
- INDIUM-111-LABELED CELLULAR BLOOD COMPONENTS - MECHANISM OF LABELING AND INTRACELLULAR LOCATION IN HUMAN NEUTROPHILS1977
- Leukocyte labeling with technetium-99mInternational Journal of Nuclear Medicine and Biology, 1976