THE DISTRIBUTION OF ACID MUCOPOLYSACCHARIDES IN COHN FRACTIONS OF HUMAN PLASMA PROTEINS1

Abstract

Conn fractions of human plasma proteins were investigated to determine the predominant site of occurrence of the acid mucopolysaccharides which can be demonstrated regularly in the euglobulin fraction of human plasma. By the methods employed, predominant occurrence was in Fractions III and IV-1, Fraction III showing the larger amount. Paper chromatographic studies of the acid mucopolysaccharide material recovered from Fractions III and IV-1 demonstrated two chief meta-chromatic components. One component resembled the chief component of commercial chondroitin sulfate in its mobility. A slower component was unidentified. Attempts to confirm or deny a chemical or physical binding between the hexuronate-containing polysaccharides and the lipo-proteins of Fractions III and IV-1 were inconclusive.