Simple methods for the purification of crude κ-casein and β-casein by treatment with calcium phosphate gel

Abstract

Summary: Batch methods applicable on a large scale are described for the purification of crude κ- and βκ-Casein, dissolved in urea-containing buffer, was freed from α_s- and β-caseins by treatment with calcium phosphate gel and recovered in about 60% yield. β-Casein was freed from most impurities by adsorption on to calcium phosphate gel at pH 7·8 in the presence of urea and elution with 6 M-urea–N-NH₄OH at 4°C. The recovery was about 50%.