Molecular Cloning of Polyoma Virus DNA in Escherichia coli : Lambda Phage Vector System
- 2 March 1979
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 203 (4383), 887-892
- https://doi.org/10.1126/science.217088
Abstract
The biological activity of recombinant phage and recombinant phage DNA containing monomeric or dimeric polyoma DNA inserts was examined in mice and cultured mouse cells. Recombinant preparations containing a single copy of viral DNA were invariably noninfectious; molecules containing a dimeric polyoma DNA insert were at least seven orders of magnitude less infectious than polyoma virions after parenteral inoculation. No infection was detected with any recombinant preparation after oral administration.This publication has 9 references indexed in Scilit:
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