COMPARISON OF 4 NEW CELL-LINES FROM PATIENTS WITH ADENOCARCINOMA OF THE OVARY

Abstract

Permanent human tumor cell lines COLO 110, COLO 316, COLO 319 and COLO 330 were established for 4 patients with serous cystadenocarcinoma of the ovary. COLO 110 was derived from primary tumor tissue; COLO 316, COLO 319 and COLO 330 were derived from cells in malignant effusions. COLO 110 and COLO 316 grew as monolayers of epithelioid cells in culture; COLO 319 and COLO 330 grew as vermiform, floating colonies of epithelioid cells in culture. Epithelial-like morphology was confirmed by transmission electron microscopy. All 4 cell lines had marker chromosomes and double minute chromosomes. Giemsa banding revealed that chromosomes 1, 3, 6 and 7 were involved in markers in all 4 lines, and chromosomes 2, 4, 5, 9, 11 and 15 were involved in markers in 3 of the cell lines. Marker chromosomes with possible homogeneous staining regions were observed in COLO 319. Estrone was elaborated by 3 of the lines, but neither chorionic gonadotropin, carcinoembryonic antigen nor estrogen, or progesterone receptor proteins were detected. Each cell line demonstrated a distinctive isozyme phenotype. These cell lines are maintained in active culture and in a cell bank for distribution to other investigators.