Rebound alkalosis and persistent lactate: Multinuclear (1H, 13C, 31P) NMR spectroscopic studies in rats

Abstract

Intracellular pH levels of infarcted brain determined by phosphorus-31 nuclear magnetic resonance (NMR) spectroscopy disclosed a notable phenomenon. The acidotic brain pH seen in the acute stage of infarction was observed to rebound into the alkalotic range in the subacute phase before returning to the normal range in the chronic phase. This “rebound alkalosis” which was usually observed between the 24th and the 48th hour after experimental induction of infarction in rats was accompanied by significant lactate levels as detected by proton NMR spectroscopy. Analysis of the satellite methyl resonance of ¹³C-lactate using high-resolution proton NMR spectroscopy after ¹³C-glucose infusion indicated that no lactate was produced in the subacute phase of infarction and that the lactate detected during this phase must have been generated prior to this phase of infarction. © 1991 Academic Press. Inc.