POT1-interacting protein PIP1: a telomere length regulator that recruits POT1 to the TIN2/TRF1 complex
Open Access
- 1 July 2004
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 18 (14), 1649-1654
- https://doi.org/10.1101/gad.1215404
Abstract
Human telomere length is controlled by a negative feedback loop based on the binding of TRF1 to double-stranded telomeric DNA. The TRF1 complex recruits POT1, a single-stranded telomeric DNA-binding protein necessary for cis-inhibition of telomerase. By mass spectrometry, we have identified a new telomeric protein, which we have named POT1-interacting protein 1 (PIP1). PIP1 bound both POT1 and the TRF1-interacting factor TIN2 and could tether POT1 to the TRF1 complex. Reduction of PIP1 or POT1 levels with shRNAs led to telomere elongation, indicating that PIP1 contributes to telomere length control through recruitment of POT1.Keywords
This publication has 21 references indexed in Scilit:
- Regulation of Telomerase by Telomeric ProteinsAnnual Review of Biochemistry, 2004
- TIN2 is a tankyrase 1 PARP modulator in the TRF1 telomere length control complexNature Genetics, 2004
- Plasma antioxidants from chocolateNature, 2003
- POT1 as a terminal transducer of TRF1 telomere length controlNature, 2003
- A Complex with Chromatin Modifiers That Occupies E2F- and Myc-Responsive Genes in G 0 CellsScience, 2002
- Targeting Assay To Study the cis Functions of Human Telomeric Proteins: Evidence for Inhibition of Telomerase by TRF1 and for Activation of Telomere Degradation by TRF2Molecular and Cellular Biology, 2002
- Senescence Induced by Altered Telomere State, Not Telomere LossScience, 2002
- Pot1, the Putative Telomere End-Binding Protein in Fission Yeast and HumansScience, 2001
- Identification of Human Rap1Cell, 2000
- A Protein-Counting Mechanism for Telomere Length Regulation in YeastScience, 1997