Detection of Adenovirus Type 12 Neoantigen(s) in a Continuous Human Amnion Cell Line (FL) by Immunofluorescence.

Abstract

Summary Neoantigens produced in FL cells by infection with oncogenic types of adenoviruses were stained by the indirect FA technic using serum from hamsters bearing tumors induced by adenovirus type 12 as the intermediate serum. The neoantigen of adenovirus type 12 first appeared at 6 hours postinfection, and thereafter the amount within the cell (nucleus) increased until 12 hours. The number of cells in which the antigen was demonstrable also increased until at 12 hours post-infection the majority of the cells contained the antigen. Formation of neoantigen preceded the formation of new infectious virus, as was shown by growth curve experiments and by staining with fluorescein-labeled antibody against the virus.