HLA‐A, ‐B, and ‐DR specificities on human monocytes

Abstract

Monocyte-enriched cell suspensions obtained by a plate adherence method from 57 blood donors were typed by microcytotoxicity for HLA-A, -B and -DR determinants. Parallel assays were performed with autologous unfractionated lymphocytes for HLA-A and -B determinants and with autologous B-lymphocytes for HLA-DR determinants. Correlation coefficients (r values) were calculated for 9 HLA-A specificites (r > 0.79), 16 HLA-B specificities (r > 0.56) and 7 HLA-DR specificities (r > 0.81). For certain HLA-A and -B specificities detection was less readily achieved on monocytes than on autologous lymphocytes. Extra reactions were observed in some instances. With sera defining HLA-DR specificities, monocytes and B-cells showed reactivity of almost identical strength and reliabiity.