Transcription factor IIA is inactivated during terminal differentiation of avian erythroid cells.
- 15 December 1992
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (24), 11678-11682
- https://doi.org/10.1073/pnas.89.24.11678
Abstract
Avian histone H5 and alpha A-globin genes are transcribed much more efficiently in whole cell extracts derived from immature polychromatic erythrocytes than in extracts from mature duck erythrocytes. We found that these differential activities are detectable only if assayed with promoters containing a functional TATA box. The addition of either highly purified human or recombinant yeast transcription factor IIA (TFIIA) to extracts from mature erythrocytes resulted in a significant increase in transcription from TATA-containing promoters, whereas transcription from TATA-less promoters remained unaffected. Moreover, the activity of TFIIA was found to be reduced in extracts from mature erythrocytes. These data support the proposition that inactivation of TFIIA may contribute to a general repression of gene activity in avian erythrocytes, and only those genes with alternative mechanisms of initiation complex formation continue to be expressed in these cells. In the case of the histone H5 gene, such an alternative mechanism could be mediated via the interaction between duck erythrocyte upstream stimulating factor and TFIID.Keywords
This publication has 26 references indexed in Scilit:
- Transcription factor eUSF is an essential component of isolated transcription complexes on the duck histone H5 gene and it mediates the interaction of TFIID with a TATA-deficient promoterJournal of Molecular Biology, 1992
- The complexities of eukaryotic transcription initiation: regulation of preinitiation complex assemblyTrends in Biochemical Sciences, 1991
- Transcription from a TATA-less promoter requires a multisubunit TFIID complex.Genes & Development, 1991
- Cooperative interaction of an initiator-binding transcription initiation factor and the helix–loop–helix activator USFNature, 1991
- Sequence-Specific Antirepression of Histone H1-Mediated Inhibition of Basal RNA Polymerase II TranscriptionScience, 1991
- A novel mediator between activator proteins and the RNA polymerase II transcription apparatusCell, 1990
- RNA POLYMERASE B (II) AND GENERAL TRANSCRIPTION FACTORSAnnual Review of Biochemistry, 1990
- Transcription of the αA‐globin gene of the duckEuropean Journal of Biochemistry, 1989
- Transcription of the chicken histone H5 gene is mediated by distinct tissue-specific elements within the promoter and the 3' enhancer.Molecular and Cellular Biology, 1989
- Conserved dyad symmetry structures at the 3′ end of H5 histone genesJournal of Molecular Biology, 1984