Lipemia Clearing by Hyaluronidase, Hyaluronate, and Deoxycorticosterone, and its Inhibition by Cortisone, Stress, and Nephrosis

Abstract

Partially depolymerized hyaluronic acid released a lipemia clearing factor following intravenous, subcutaneous and oral administration to rats. Hyaluronidase and desoxycorticosterone (DCA) which were administered only to rats hypodermically were also effective. Plasma from rats treated with cortisone prevented the lipemia clearing by heparin, DCA and partially depolymerized hyaluronic acid. The effect of exposure to cold was tested only against heparin lipemia clearing and was also found to inhibit it. Release of lipemia clearing inhibitor appears to be another manifestation of the general adaptation syndrome. Plasma from rats made nephrotic by administration of low doses of anti-kidney serum had in addition gross lipemia suggesting that pathological lipemia may be preceded by the appearance of the inhibitor. The lipemia clearing factor was inactivated by incubation with hyaluronidase in vitro suggesting that this factor contains a mucopolysaccharide moiety which is not heparin. The activity of the inhibitor of the lipemia clearing factor was not affected by heating at 60[degree] C for 1 hour.