Plasmid stability in budding yeast populations: Steady-state growth with selection pressure

Abstract

Plasmid gene product accumulation in a cell population depends on the fraction of plasmid‐containing cells and the distribution of single‐cell plasmid content. These important population properties have been related to plasmid replication regulation and kinetics and to plasmid segregation rules at the single‐cell level using population balance mathematical models. Budding yeast populations are considered in detail because of the practical potential of yeast host–vector systems and because of the model complications introduced by the asymmetric division pattern observed for Saccharomyces cerevisiae at all but the largest growth rates. Solutions are presented for several different reasonable models of plasmid replication and segregation. The results offer potential for identification of important qualitative features of yeast plasmid replication and of model parameter values from average and segregated experimental data on yeast populations.