Lethal Effect of Certain Benzimidazoles and Benzenes on Early Chick Embryo.
- 1 October 1958
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 99 (1), 232-235
- https://doi.org/10.3181/00379727-99-24305
Abstract
The lethality of 7 compounds structurally related to vitamin B12 was determined by injecting several doses of each compound into the albumen prior to incubation. The lethal index (percent dead/uM of inhibitor/egg) for each compound was: (I) methylamide, 0.5; (II) l,2-dimethyl-4,5-diaminobeneene, 2.8; (HI) 5,6-dimethylbenzimidazole, 3.7; (IV) 2,5-dimethylbenzimidazole, 8.0; (V) l,2-dichloro-4,5-diaminobenzene, 9.5; (VI) 2-ethyl-5-methyl-benzimidazole, 12.6 (injected Oct.-Nov., random bred, White Leghorn); (VII) 2-hepta-5-methylbenzimidazole, 15.5; and (VI) 2-ethyl-5-methyl-benzimidazole, 29.0 (injected Feb.-March, Mt. Hope, White Leghorn). The effect of vitamin B12 was tested on compounds V, VI, VII; it alone was non lethal and failed to influence the lethality of V and VII. However, high levels partially reversed VI under conditions which varied with seasonal and/or genetic differences. The lethality of the VI also varied under these conditions. The relative order of lethality of the benzimidazoles was the same as in virus inhibitory work, (Tamm et al., J. Exp. Med. 98: 245. 1953), while that of the benzenes was the same as the inhibition of microorganisms which synthesize B12 and/or riboflavin (Woolley, Proc. Soc. Exp. Biol. Med. 75: 745. 1950). The 3 inhibitors which are natural moities of vitamin B12, I, II, and III, were the least lethal of the compounds tested. The implications of these findings were discussed.Keywords
This publication has 9 references indexed in Scilit:
- The distribution of vitamin B12 in the developing chick eggArchives of Biochemistry and Biophysics, 1958
- The temporary inactivation of newt larvae by benzimidazole and its alkyl derivativesCellular and Molecular Life Sciences, 1958
- Effect of Antimetabolites on Growth of Endamoeba histolytica.III. 5,6-dimethylbenzimidazole and Related Compounds.Experimental Biology and Medicine, 1957
- CERTAIN BENZIMIDAZOLES, BENZENES, AND RIBOFURANOSYLPURINES AS INHIBITORS OF INFLUENZA B VIRUS MULTIPLICATIONJournal of Bacteriology, 1956
- Inhibition of in vitro Heme Synthesis from N15-Glycine by 2-Ethyl-5-methylbenzimidazole.,Experimental Biology and Medicine, 1954
- INHIBITION OF INFLUENZA VIRUS MULTIPLICATION BY ALKYL DERIVATIVES OF BENZIMIDAZOLEThe Journal of Experimental Medicine, 1953
- SELECTIVE TOXICITY OF 1,2-DICHLORO-4,5-DIAMINOBENZENE: ITS RELATION TO REQUIREMENTS FOR RIBOFLAVIN AND VITAMIN B12The Journal of Experimental Medicine, 1951
- Inhibition of Synthesis of Vitamin B12 and of Riboflavin by 1,2-Dichloro-4, 5-diaminobenzene in Bacterial CulturesExperimental Biology and Medicine, 1950
- Congenital Anomalies in the Chick Due to Vitamin B12 Deficiency , , , ,Journal of Nutrition, 1950