PURIFICATION OF GONADOTROPHIN FROM HUMAN MENOPAUSAL URINE
- 1 March 1964
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 45 (3), 321-328
- https://doi.org/10.1530/acta.0.0450321
Abstract
Gonadotrophins were prepared from human postmenopausal urine with the kaolin-acetone method and purified by adsorption of impurities on diethylamine ethyl cellulose (DEAE-C). The last step, namely chromatography on the column of permutit, resulted in a significantly improved specific activity. The immuno-electrophoresis showed that the purified HMG is not homogeneous and that the constituents are glycoproteins which migrate into the region a1, a2 and [beta] globulins.Keywords
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