Abstract
Glutamine is not required for staphylococcal growth, and was found to be synthesized by staphylococci from NH3 and glutamic acid in the presence of glucose. The amt. of glutamine found was, however, much less than that of the NH3 reacting. Similar relationships were found in Proteus morganii, P. vulgaris, Saccharomyces cerevisiae, and S. ellipsoideus, P. morganii was outstanding in possessing a powerful glutaminase which could easily be separated from the cells; in this it differed markedly from P. vulgaris. Glutaminase activity was also demonstrated in yeasts, where its occurrence has been previously a matter of dispute.