THE EFFECTS OF THE ARYLSULFONYLUREAS ON THE RESPIRATORY ACTIVITY AND GLUCOSE METABOLISM OF ISOLATED RABBIT KIDNEY CORTEX1

Abstract

Slices of kidney cortex from fed and from fasted rabbits were incubated in the Warburg apparatus in glucose-free and glucose-containing phosphate-buffered media. The addition of 5xl0-3 [image] Orinase in vitro effected an increase in cellular hydration and a profound reduction in oxygen consumption. Net disappearance of stable and radioactive glucose from the suspending medium was increased in five of six experiments. In all studies with 5xlO-3 [image] Orinase, significantly smaller proportions of the assimilated glucose-C14 were incorporated into tissue lipids or recovered as radioactive CO2. Under similar conditions, the metabolic product of Orinase, p-carboxyphenylsulfonylurea, was less than one-fifth as potent an inhibitor of the respiratory metabolism of surviving rabbit kidney cortex.