A Growth-Promoting Substance for L. bulgaricus 09 in Whey: Isolation and Identification as Orotic Acid

Abstract
Various natural substances, especially whey, were found to contain a substance required for growth of Lactobacillus bulgaricus 09. In the survey of known compounds orotic acid was found to satisfy the requirement of this organism for natural material. The micro-biologically active material was isolated from one of the natural sources, whey, and identified as orotic acid.